FIGURE 2.
Effect of AAV-mediated short-hairpin virus knockdown of PAC1R in the NAcc Shell in Scr:sP rats on ethanol self-administration in an FR-1 schedule of reinforcement (n = 8/group). (A,B) Rats with NAcc Shell PAC1R shRNA knockdown (“AAV-PAC1R KD”) drank significantly more ethanol than control AAV rats (“AAV-CTRL”). (C,D) NAcc Shell AAV-PAC1R KD rats drank less water in later sessions. (E,F) NAcc Shell AAV-PAC1R KD rats also showed a higher preference for ethanol compared to AAV-CTRL rats. Data represent Mean ± SEM. ∗p ≤ 0.05, ∗∗p ≤ 0.01.