TABLE 3.

Sulforaphane effects on the lifespan of C. elegans strains.

C. elegans strains	Temperature (°C)	Fig. No.	Exp. No.	Sulforaphane (μM)	Mean lifespan ±SEM (days)	%, a Mean increase (SF treatment)	p Value
N2	20	1B	1	0	16.19 ± 0.52	15.5	0.001
				100	18.70 ± 0.53
			2	0	20.99 ± 0.52	8.9	0.037
				100	22.85 ± 0.47
			3	0	19.47 ± 0.53	9.9	0.024
				100	21.40 ± 0.52
N2 (tra-1i)	20	1D	1	0	9.89 ± 0.41	—	0.485
				100	9.55 ± 0.37
			2	0	9.62 ± 0.31	—	0.839
				100	9.65 ± 0.31
			3	0	9.09 ± 0.41	—	0.103
				100	10.24 ± 0.59
CB4270	15	1E	1	0	19.70 ± 0.79	—	0.352
				100	18.43 ± 0.80
			2	0	28.45 ± 0.89	—	0.710
				100	27.22 ± 0.90
			3	0	27.88 ± 1.08	—	0.766
				100	28.20 ± 1.04

^aMean lifespan: Corresponds to the mean lifespan as measured in the present study.

Summary and statistical analysis of sulforaphane effects on lifespan experiments. N2: Wild-type C. elegans strain; N2 (tra-1i): loss-of-function tra-1 C. elegans strain; CB4270: tra-1(−) mutant C. elegans strain. The mean lifespan values were analyzed by log-rank (Kaplan–Meier) statistical tests. p values < 0.05 were considered statistically significant. p values were calculated for individual experiments (n = 100). All statistical evaluations were calculated using SPSS 25.0. (−) not calculated because the standard deviation was >0.05.

SEM: standard error of the mean.