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. 2021 Dec 3;9:689067. doi: 10.3389/fcell.2021.689067

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Copyright © 2021 Zhang, Liang, Wu, Pei, Guo, Chu, Ding, Bao, Kalwar and Yan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Preadipocyte-induced differentiation and detection of m⁶A level between preadipocyte and adipocyte. (A,B) Preadipocytes and adipocytes dyed with Oil red O. (C) Relative expression of PPARγ, C/EBPα, and FABP4 between preadipocyte (day 0) and adipocyte (day 12). (D) The mRNA expression level of methyltransferases (METTL14, WTAP, and METTL3), demethylases (FTO and ALKBH5) and m⁶A-binding proteins (YTHDC1and YTHDC2). (E) m⁶A abundance between the preadipocytes and adipocytes. (SEM, * p < 0.05;** p < 0.01).