TABLE 1.
Primers used in this study and PCR conditions.
| Target | Primer name | Sequence (5′–3′)a | Use | Tmb | Reference |
| adeABC | P_adeA_F | ATTCGGGATCCCTTCATTTGGGTTAAAAGGCTTC | Promoter amplification | 59.67 | This study |
| P_adeA_R | ATTCGGCGGCCGCCATACTGTCCAAACCTAGTGAGTTT | 57.02 | This study | ||
| adeFGH | P_adeF_F | ATTCGGGATCCCGATACAGGCACATCAATACGA | Promoter amplification | 58.77 | This study |
| P_adeF_R | ATTCGGCGGCCGCAGGTGCTCCTAGTTATTTGGATACC | 59.85 | This study | ||
| adeIJK | P_adeI_F | ATTCGGGATCCTTGCACGCGTAGGCGG | Promoter amplification | 60.02 | This study |
| P_adeI_R | ATTCGGCGGCCGCGTTCCACCTCGTTTAGATAAAATCTA | 58.33 | This study | ||
| adeRS | P_adeR_F | ATTCGGGATCCCTTTGAGTCTTGCTACCTCAGCTT | Promoter amplification | 59.53 | This study |
| P_adeR_R | ATTCGGCGGCCGCGATAATCTGGCTATAGAAAGTGCTTC | 58.13 | This study | ||
| pLPV1Z | T3 (modified) | GCAATTAACCCTCACTAAAGG | Constructs confirmation | 54.09 | Lucidi et al., 2019 |
| RBS | CTTAATTTCTCCTCTTTACTTACTT | 51.46 | This study |
aBoldface target of the BamHI enzyme in forward (F) primers and of the NotI enzyme in reverse (R) primers, respectively.
Italics nucleotides indicate a random sequence included to facilitate the action of the enzyme.
bTo calculate Tm, additional nucleotides to facilitate cleavage by restriction enzymes were not included.