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. 2021 Dec 3;12:711905. doi: 10.3389/fmicb.2021.711905

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Copyright © 2021 Liu, Zeng, Qu, Wang and Ning.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The microbial community structure in the ileum was significantly altered by adding CE (n = 6 hens/group). (A) Species accumulation curve is used to estimate the rationality of sequencing sample quantity. (B) Alpha diversity Rarefaction curve based on Good’s Coverage value, which reflects the rationale of sequencing depth. (C) Alpha-diversity evaluation of ileum microbial richness and evenness by measuring chao1, observe-species, Shannon, and Simpson’s diversity indexes. (D) Venn diagram is used to represent the amount of shared and unique OTUs numbers. (E) Principal coordinate analysis (PCoA) is used to determine the similarities of microbial communities between different groups. *P < 0.05 compared to two groups.