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. 2021 Dec 1;12:761922. doi: 10.3389/fphar.2021.761922

FIGURE 1.

FIGURE 1

Effects of IL-1β on knee chondrocytes in mice. (A) Mice chondrocytes were treated with various concentration gradients of IL-1β (0, 1, 2, 5, and 10 ng/ml) for 24 h, (B) or with IL-1β at the concentration of 5 ng/ml for different time points (0, 1, 6, 12, 24, and 48 h), and cell viability was detected with a CCK-8 kit. (C) Western blots and (E) quantitative analysis of aggrecan, collagen II, iNOS, MMP3, and MMP13 for chondrocytes exposed to different concentrations of IL-1β (0, 1, 2, 5, and 10 ng/ml) for 24 h. (D) Western blotting results and (F) quantitative analysis of aggrecan, collagen II, iNOS, MMP3, and MMP13 for chondrocytes stimulated with IL-1β (5 ng/ml) at several time points (0, 1, 6, 12, 24, and 48 h). GAPDH was used as an internal reference. Data are presented as means ± SD (n = 3). The exact p value was marked in the corresponding figure, N.S. indicated no significance, and p < 0.05 was considered statistically significant.