FIGURE 2.

Effects of PA on cell viability and PA suppressed inflammatory responses induced by IL-1β. (A) Molecular structure of PA. (B) Mice chondrocytes were treated with PA (0, 1.25, 2.5, 5, 10, 20, and 40 μM) for 24 and 48 h, and cell viability was detected with a CCK-8 kit. (C–G) Toluidine blue staining of chondrocytes treated with IL-1β (5 ng/ml) and PA for 24 h (scale bar 200 μm). (H) Western blotting results and (I,J) quantitative analysis of COX2 and iNOS in IL-1β–induced chondrocytes treated with PA. GAPDH was used as an internal reference. Data are presented as means ± SD (n = 3). The exact p value was marked in the corresponding figure and p < 0.05 was considered statistically significant.