Table 2.

Theoretical and measured mass of intact HB0025.

Peak	Glycosylation	Theoretical Mass (kDa)	Measured Mass (kDa)	Mass error (kDa)
Peak 1	G2FS1(4)/G0F/G1F	182131.1	182134.2	3.1
	G2FS1(4)/G1F(2)	182293.2	182289.5	-3.7
	G2FS1(3)/G2FS2/G0F/G1F	182422.4	182426.2	3.8
	G2FS1(3)/G2FS2/G1F(2)	182584.5	182585.2	0.7
Peak 2	G2FS1(3)/G0F/G1F	180070.3	180073.6	3.3
	G2FS1(3)/G1F(2)	180232.4	180217.2	-15.2
	G2FS1(2)/G2FS2/G0F/G1F	180361.5	180366.4	4.9
	G2FS1(2)/G2FS2/G1F(2)	180523.6	180512.8	-10.8
Peak 3	G2FS1(2)/G0F/G1F	178009.4	178014.0	4.6
	G2FS1(2)/G1F(2)	178171.6	178169.8	-1.8
	G2FS1/G2FS2/G0F/G1F	178300.6	178306.7	6.1
	G2FS1/G2FS2/G1F(2)	178462.8	178467.1	4.3

(2), (3), and (4) indicate that there are 2, 3, and 4 N-glycan.