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. 2021 Dec 7;65(4):3237. doi: 10.4081/ejh.2021.3237

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This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — CGN knockout mediates the proliferation, apoptosis, migration, and invasion of OS cells induced by the miR-1270 downregulation. A) The expression of CGN. B) OS cell viability of OS cells determined by CCK-8 assay. C) The proliferation of OS cells determined by colony formation assay. D) The apoptosis of OS determined by TUNEL assay. E) The apoptosis rates determined by flow cytometry. F) The expression of Bcl-2, BAX, cleaved-Caspase-3, and cleaved-Caspase-9 determined by Western blot. G,H) The migration and invasion ability determined by transwell assay (magnification: 100x). **p<0.01 vs NC inhibitor group; ##p<0.01 vs miR-1270 inhibitor group.