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. 2021 Nov 16;32(12):2507–2515. doi: 10.1021/acs.bioconjchem.1c00473

Figure 4.

Figure 4

(A) Chromatogram of cell medium before (solid line) and after (dashed line) click reaction between XylNapN3-primed GAGs and an Alexa Fluor 647 fluorophore. The broad peak between 5 and 10 min corresponds to xyloside-primed GAGs. The tall peak at 12 min corresponds to the excess fluorophore. Fluorescence was monitored at Ex/Em = 648/671 nm. (B) SPR sensorgram showing the interaction between XylNapN3-primed HS GAGs and hepatocyte growth factor (HGF) at 3, 6, and 12 nM. Black curves show fitting using a 1:1 Langmuir model. (C) Confocal microscopy images of GFP-tagged A549 cells treated with XylNapN3–TAMRA conjugates. The compound is efficiently taken up by the cells and localizes to the perinuclear region.