FIG. 2.

Mapping of the cDNA 5′-terminal sequences to the L1Hs 5′UTR. (A) cDNA sequences with the mRNA sequence polarity (5′ to 3′) indicated by arrows were mapped to the L1Hs 5′UTR. Numbering of the top L1Hs strand starts with +1 (34), and numbering of the bottom strand starts from nt 86 of ORF1. Splicing of the N4 and P3 cDNAs within the 5′UTR is indicated by thin lines. Two cDNAs (N9 and P2) showed opposite polarity. (B) Six cDNA sequences (N4, N5, N7, N12, P1, and P3) compared to the corresponding genomic sequences (Table 1) were aligned to show their exon and intron structures, indicated by upper- and lowercase letters, respectively. The conserved 5′ and 3′ intronic dinucleotide sequences (GT and AG) are underlined. Two cDNAs (N4 and P3) are spliced within the L1Hs 5′UTR, and their identical exon II sequences compared to the corresponding L1 sequence are boxed. The others are spliced to known or unknown sequences located further upstream of the L1Hs. Numbering is according to the L1Hs 5′UTR bottom strand. L1.2, accession no. M80343 (8). Identical nucleotides are marked below sequences by asterisks; sequences not shown are indicated by dots.