TABLE 1.
Sequencing and mapping data of 15 cDNAs isolated from the NTera2D1 library
Clone | Lengtha (bp) | Similarity to L1.2 5′UTRb
|
Similarity/identity to known gene or regionc | BAC clone (accession no.) | Chrd | No. of exonse | Promoter distancef (kb) |
---|---|---|---|---|---|---|---|
Region (bp) % | |||||||
N1 | 2,181 | 1–163 ≡ 163–2 95 | 171–284—Alu | AC020581 | 7 | 4 | >12 |
345–778—Tigger2a | AC009264 | ||||||
1752–2004—MIR | |||||||
1882–2161—AA084761 (EST) | |||||||
N4 | 1,205 | 10–211 ≡ 461–261 90 | GAP-43 mRNA (NM_002045) | AC021376 | 3 | 4 | >50 |
210–324 ≡ 117–4 98 | 362–1205 = 122–965 | AC012565 | |||||
N5 | 1,664 | 1–156 ≡ 414–260 94 | 398–508—Alu | AC010148 | NDg | 4 | ∼1 |
782–1151/1361–1664—MER4 | |||||||
N7 | 1,670 | 1–190 ≡ 449–260 97 | 192–677—Hyp protein (47%) (Q12765) | AC018470 | 2 | 5 | >4 |
N8 | 762 | 1–326 ≡ 329–5 95 | 339–762—L1 (M80343) | ND | ND | ND | ND |
N9 | 1,956 | 1050–1632 ≡ 769–175 81 | mRNA, homologue of mouse | AL022400 | 1 | 9 | ND |
1636–1771 ≡ 146–12 90 | Hh1 mRNA (AL157958) | AL022171 | |||||
1–64 ≡ 913–976/63–989 ≡ 1011–1847 (99%) | AL021069 | ||||||
N10 | 1,930 | 1–450 ≡ 456–7 98 | CHRM3 gene (U29589) | AC015901 | 1 | 5 | >62 |
760–1922 ≡ 183–1345 | AC013517 | ||||||
N11 | 1,777 | 1–104 ≡ 449–346 97 | 103–244—FAM | AC015495 | 3 | ≥2 | >15 |
403–473—MIR | |||||||
1368–1777—AW513672 (EST) | |||||||
N12 | 441 | 1–177 ≡ 434–259 93 | SPT3 mRNA (AF073930) | AL138880 | 6 | ≥4 | >40 |
176–441 ≡ 173–438 | AL161905 | ||||||
L2 | ∼2,000 | 1–109 ≡ 453–346 94 | 105–280/651–893/3′ end—L1 | AC019310 | 4 | ND | ND |
469–527—MIR | |||||||
P1 | 1,687 | 1–194 ≡ 455–261 93 | 189–292—MLT | AC023485 | 3 | ≥5 | >9 |
1026–1326/1373–1477—MER4 | |||||||
P2 | ∼3,500 | 1–122 ≡ 23–142 93 | 5′ and 3′ ends/396–524—Alu | ND | ND | ND | ND |
275–395—Tigger1 | |||||||
P3 | 1,818 | 1–221 ≡ 481–261 92 | 361–460/488–816/876–1320/1502–1616—MLT | AC021696 | >9 | >7 | |
P5 | 2,881 | 1–429 ≡ 428–1 93 | OATP mRNA (U21943) | AC006559 | 12 | 16 | ∼60 |
669–2881 ≡ 1–2213 | AC022224 | ||||||
P6 | ∼3,000 | 3–152 ≡ 153–5 96 | 223–319—MLT | ND | ND | ND | ND |
Complete and partial sequences (5′ and 3′ ends) of individual cDNAs are noted by exact and approximate sizes, respectively.
Similarity between regions of cDNA and L1.2 5′UTR (accession no. M80343) determined by BLAST2 sequences comparison and indicated by ≡ sign.
cDNA sequences similar to DNA repeats or EST sequences and identical to the corresponding regions of known genes (indicated after long dashes) are shown. Accession and ESTs numbers are indicated in parentheses.
Chr, chromosome number determined from BAC location.
Determined from cDNA and BAC sequence comparison using BLAST2.
Approximate distance of the ASP from the coding sequence is shown because of gaps and unordered fragments in unfinished BACs.
ND, not determined.