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DES-PUU-N (approximately 54 kDa) immunoblotted with a pool of PUU N-reactive MAbs (MAbs 1C12, 4C3, 5B5, and 3G5) after transient transfection of various amounts of pAc5.1/V5-His/Kaz-S to equal numbers of S2 cells. Lane 1, 10 μg; lane 2, 20 μg; lane 3, 30 μg; lane 4, 40 μg; lane 5, negative control (S2 cells); lane 6, positive control (native PUU N antigen). Molecular masses (in kilodaltons) are indicated to the right.
