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IgG ELISA titration curves. Results for known concentrations of purified rN-Kaz (⧫) were compared to those for dilution series (starting from 1:10 with twofold dilutions) of crude extracts of DES-PUU-N (■; stable transfectants harvested from one 225-cm2 cell culture flask and sonicated in 5 ml) and native PUU N (▴; infected cells from five 800-cm2 roller bottles and sonicated in 5 ml). OD, optical density at 405 nm.
