Figure 2.

Wound-draining lymph nodes, but not the spleen, contain Th17 cells 3 days and 14 days after burn injury. Cells were harvested from spleens, as well as inguinal and axillary PLNs, 3 days or 14 days after a 20% TBSA, full-thickness burn, or sham injury. Bulk PLN cells were stimulated in vitro and intracellular cytokine staining was performed, and flow cytometry was used to measure IL-17 and IFN-γ production by CD3⁺ CD4⁺ cells. Representative examples of the flow cytometry histograms for each lymphoid organ are provided.