Figure 3.

Percentage of CD4⁺ T cells that produce IL-17 are significantly higher in burn compared with sham. Cells were harvested from spleens, as well as inguinal and axillary PLNs, 3 days or 14 days after a 20% TBSA, full-thickness burn, or sham injury. Bulk PLN cells were stimulated in vitro and intracellular cytokine staining was performed, and flow cytometry was used to measure IL-17 and IFN-γ production by CD3⁺ CD4⁺ T cells. Data were expressed as mean ± standard error of the mean, *p ≤ 0.05; **p ≤ 0.005 compared with matched sham controls by Student’s t test (n = 4–6 mice/group).