Figure 5.

CD4⁺ T cells that produce IL-17 also express ROR-γt. Cells were harvested from inguinal and axillary PLN, 7 days or 14 days after a 20% TBSA, full-thickness burn injury. CD4⁺ T cells were enriched by negative magnetic selection and stimulated in vitro. Intracellular cytokine and ROR-γt staining was performed, and flow cytometry was used to gate Th17 (IL-17⁺ CD4⁺) and Th1 (IFN-g⁺ CD4⁺) T cells. Representative ROR-γt staining is shown in each gated population. Numbers represent mean ROR-γt fluorescent units ± standard error of the mean, *p ≤ 0.05; compared with matched isotype control by Student’s t test (n = 4–6 mice/group).