Figure 6.

Th17/Th1 ratio is dynamic after burn injury. Cells were harvested from various PLNs at 3 days, 7 days, or 14 days after a 20% TBSA, full-thickness burn, or sham injury. Negative magnetic selection was performed to enrich the CD4⁺ T-cell population. CD4⁺ cells were stimulated in vitro before intracellular cytokine staining was performed, and flow cytometry was used to quantify IL-17⁺ CD4⁺ T cells. For each time point, the average sham Th17/Th1 ratio was set at 1.0. For each burn mouse, its Th17/Th1 ratio was normalized to the corresponding sham ratio. A ratio >1.0 represents a skew in the proinflammatory response to a Th17 phenotype, whereas a ratio below 1.0 indicates that a Th1 phenotype is dominant. ***p ≤ 0.0005 compared with matched sham controls by Student’s t test (n = 4–6 mice/group).