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. Author manuscript; available in PMC: 2021 Dec 17.
Published in final edited form as: J Trauma. 2011 Mar;70(3):681–690. doi: 10.1097/TA.0b013e31820d18a6

Figure 7.

Figure 7.

CD4+ T cells from burn and sham mice have similar abilities to polarize to Th17 cells. Cells were harvested from various PLNs at 3 days or 14 days after a 20% TBSA, full-thickness burn, or sham injury. CD4+ T cells were enriched by negative magnetic selection and cultured with plate bound anti-CD3, soluble anti-CD28, IL-6, and TGF-β for 4 days. IL-17 and IFN-γ production by CD3+ CD4+ cells was analyzed using flow cytometric staining. Representative examples of the histograms for each timepoint are provided.