TABLE 2.
Oligonucleotide used in plasmid or strain construction
| PCR product | Oligonucleotide sequence (5′→3′) |
|---|---|
| RAD23 ORF for GAL1 expression (5′, BamHI site) | CGGGATCCAAAAATGGTTAGCTTAACCTTT |
| RAD23 ORF for GAL1 expression (3′, BamHI site) | CGGGATCCAATCTCAGTCGGCATGATCGCT |
| DDI1 ORF for GAL1 expression (5′, BamHI site) | CGGGATCCAAAGATGGATTTAACAATTTCA |
| DDI1 ORF for GAL1 expression (3′, BamHI site) | CGGGATCCTTGATCATTGGAAAAGGAGGGA |
| Rad23p C terminus for tagging (5′, SalI site) | GTCGACCATCCGGTGCGCTTGGCACAACTG |
| Rad23p C terminus for tagging (3′, NotI site) | GCGGCCGCCGTCGGCATGATCGCTGAATAG |
| Ddi1p C terminus for tagging (5′, SalI site) | GTCGACAATTATTGGGAGAATTCACCAAGC |
| Ddi1p C terminus for tagging (3′, NotI site) | GCGGCCGCCTTGGAAAAGGAGGGATGCAGC |
| Radp23p ΔUBA2 C terminus for tagging (3′, NotI site) | GCGGCCTCAATAGTCAACTTGGAAAGAACCTTC |
| Ddi1p ΔUBA C terminus for tagging (3′, NotI site) | GCGGCCTCACGTTGTAGCACCGGCTGTACTTCT |