TABLE 3.
RSA azole MICs determined with diluted inoculum and prolonged incubation
| Isolate no. | Species | Drug | MIC (μg/ml) determined by:
|
||
|---|---|---|---|---|---|
| 48- NCCLS | RSA
|
||||
| Undiluted, 6 ha | Diluted, 19 hb | ||||
| 97-049 | C. glabrata | FLU | 64 | >64 | 64 |
| ITRA | 2 | >8 | 2 | ||
| 97-050 | C. glabrata | FLU | 8 | >64 | 8 |
| ITRA | 1 | >8 | 0.5 | ||
| 97-055 | C. tropicalis | FLU | 1 | >64 | 0.25 |
| ITRA | 0.125 | 0.125 | 0.125 | ||
| 97-012 | C. albicans | FLU | 4 | >64 | 8 |
| ITRA | 0.5 | 0.25 | 0.5 | ||
| 97-056 | C. parapsilosis | FLU | 0.25 | 4 | 0.5 |
| ITRA | 0.125 | 0.125 | 0.125 | ||
a
In the RSA, an inoculum with a turbidity of an 0.5 McFarland standard was used initially without dilution, with an incubation time of 6 h. Various inoculum dilutions and incubation times were subsequently tested for the azole compounds.
b
The original 0.5-McFarland standard inoculum preparation was diluted 1:1,000 in RPMI 1640 medium without glucose before inoculation. Plates were incubated for a total of 19 h before the complete color mixture was added.