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. 2021 Dec 15;219(2):e20211336. doi: 10.1084/jem.20211336

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© 2021 Lee et al.

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Figure 3. — CD4⁺ T cell–intrinsic TBK1 is dispensable for Tfh cell maturation. (A) Depletion efficiency of Tbk1 in splenic CD4⁺ T cells of Cd4^cre Tbk1^f/f mice (T Tbk1^−/−) and Cd4^wt Tbk1^f/f mice (T Tbk1^+/+) was analyzed by immunoblotting. B cells from WT mice were used as a control. (B) Population of Tfh cells in the spleens of T Tbk1^−/− and T Tbk1^+/+ mice on day 24 after infection with PyNL analyzed by FACS. n = 4–5 mice/group. (C) Representative FACS plots and histograms of BCL6, IL-21, and ICOS expression in naive T cells, activated T cells, pre-Tfh cells, and mature Tfh cells in the spleens of T Tbk1^−/− and T Tbk1^+/+ mice on day 24 after infection with PyNL. n = 4–5 mice/group. MFI, mean fluorescence intensity. (D) Population of Tfh cells in the spleens of T Tbk1^−/− and T Tbk1^+/+ mice on day 12 after immunization with NIP-OVA with alum. n = 5 mice/group. (E) GC population in the spleens of T Tbk1^−/− and T Tbk1^+/+ mice on day 24 after infection with PyNL. n = 4–5 mice/group. (F) GC population in the spleens of T Tbk1^−/− and T Tbk1^+/+ mice on day 12 after immunization with NIP-OVA with alum. N = 5 mice/group. Each dot represents an individual mouse. Data are representative of two independent experiments (A–F). Data are shown as mean ± SD. Mann-Whitney t test (B–F). Source data are available for this figure: SourceData F3.