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. 2021 Dec 15;219(2):e20211336. doi: 10.1084/jem.20211336

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© 2021 Lee et al.

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Figure 6. — TBK1 is a limiting factor that fine tunes CD40 and BCR signaling in Pre-GC. (A) Schematic diagram of ex vivo splenic B cells isolated from day 9 PyNL-infected B Tbk1^−/− and B Tbk1^+/+ mice with CD40 and/or BCR stimulation for the evaluation of IRF4 and c-Myc expression on gated B220⁺ GL7⁺ Pre-GC and B220⁺ GL7⁻ non–Pre-GC B cell population by FACS. (B and C) Representative histograms and graphs showing IRF4 expression (B) and c-Myc expression (C) of GL7⁺ Pre-GC and GL7⁻ non–Pre-GC upon CD40 and/or BCR stimulation. n = 4 mice/group. (D) Representative histograms and graph showing AKT phosphorylation at S473 and T308 on Pre-GC cells on day 9 after infection with PyNL. n = 4 mice/group. Each dot represents data from an individual mouse. Data are representative of two independent experiments (A–D). Data are shown as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; one-way ANOVA (B and C), Mann-Whitney t test (D). gMFI, geometric mean fluorescence intensity.