FIGURE 4.

Noncanonical Itk ^–/− Tregs suppress serum level inflammatory cytokine production by donor T cells. (A‐F) 1 × 10⁶ purified WT CD3⁺ T cells were transplanted with _TCDBM into irradiated BALB/c mice. At day 7 post allo‐HSCT, recipient BALB/c were euthanized and serum cytokines (IFN‐γ, TNF‐α, IL17A, IL‐5, IL‐2 and TGF‐β were determined by multiplex ELISA. (G) IFN‐γ expression by donor CD8⁺ T cells taken from recipient spleen 7 days post‐transplant. (H) TNF‐α expression by donor CD8⁺ T cells taken from recipient spleen 7 days post‐transplant. Combined data from two independent experiments is shown for cytokine restimulation. Statistical analysis was performed using one‐way ANOVA with Tukey's test, p value presented with each figure. Symbol meaning for p values are: ns, p > .05; * p ≤ .05; ** p ≤ .01; *** p ≤ .001; **** p ≤ .0001. One experiment's data are shown as representative from three independent experiments for serum ELISA