Table 3.
Correlations between methylation-based CRP scores and circulating log-CRP level, log-IL6 level, peripheral blood leukocyte types, BMI, and smoking score residual among controls only
| Score 1b | Score 2c | Score 3d | |
|---|---|---|---|
| Spearman (p value) | Spearman (p value) | Spearman (p value) | |
| Pancreatic cancer study (controls only) | |||
| Log-CRP levela | 0.284 (4.27e−06) | 0.247 (6.96e−05) | 0.297 (1.56e−06) |
| Log-IL6 levela | 0.213 (7.53e−04) | 0.158 (1.32e−02) | 0.203 (1.33e−03) |
| CLUE I/II (controls only) | |||
| CD4T | 0.425 (1.65e−10) | 0.359 (1.00e−07) | 0.212 (2.13e−03) |
| CD8T | 0.504 (8.78e−15) | 0.461 (2.34e−12) | 0.390 (5.74e−09) |
| NK | 0.062 (3.70e−01) | 0.175 (1.17e−02) | 0.026 (7.09e−01) |
| B cell | 0.103 (1.38e−01) | 0.069 (3.23e−01) | − 0.027 (6.99e−01) |
| Neutrophils | − 0.467 (1.19e−12) | − 0.422 (2.24e−10) | − 0.264 (1.17e−04) |
| Monocytes | − 0.292 (1.92e−05) | − 0.330 (1.17e−06) | − 0.288 (2.38e−05) |
| mdNLR | − 0.488 (7.66e−14) | − 0.449 (1.01e−11) | − 0.281 (3.96e−05) |
| BMI | 0.025 (7.22e−01) | 0.070 (3.12e−01) | − 0.063 (3.68e−01) |
| Methylation-predicted pack-years residuale | 0.475 (4.46e−13) | 0.375 (2.43e−08) | 0.632 (1.42e−24) |
aCorrelations with log-CRP level and log-IL6 level were tested with a pancreatic cancer dataset[43]
bCpG Score 1 is built using 54 CpG sites
cCpG Score 2 is built using the top 10 highly cell-specific CpG sites
dCpG Score 3 is built using the 10 modestly cell-specific CpG sites
eWe calculated a pack-years methylation score to represent pack-years smoked associated methylation alterations. This score correlates with gene expression changes that are affected by smoking