Fig. 3.

CiRNAseq RNA quantification capacity mirrors bacterial growth and activity. A The OD obtained from monitoring E. coli growth for 48 h reveals the bacterial growth phases. The nine orange time points indicate the phases from when samples were taken for sequencing analyses. B E. coli URC correlated with the OD, particularly from the lag to the exponential phases. Samples taken in the stationary phase had lower URC than the last measurement within the exponential phase. C RNA concentrations of the samples taken for sequencing are parallel to the OD, indicating that low URC found in time points six and seven may reflect the measurement of ribosomal activity. D RNA concentrations also match the URC obtained from sequencing