TABLE 2.

Steady-state kinetic parameters for nucleotide incorporation by the wild-type Rad30 protein and the E156A mutant Rad30 protein

Template and protein	dNTP	kcat (min−1)	Km (μM)	kcat/Km ratio (μM−1 min−1)	Relative efficiency
G
Wild type	dCTP	1 ± 0.09	0.06 ± 0.02	50	1
E156A	dCTP	0.1 ± 0.006	10 ± 2	0.01	2 × 10−4
A
Wild type	dTTP	1 ± 0.07	0.07 ± 0.01	20	1
E156A	dTTP	0.03 ± 0.001	20 ± 3	2 × 10−3	1 × 10−4
T
Wild type	dATP	1 ± 0.06	0.03 ± 0.01	30	1
E156A	dATP	0.07 ± 0.008	200 ± 40	4 × 10−4	1 × 10−5
C
Wild type	dGTP	1 ± 0.07	0.02 ± 0.005	70	1
E156A	dGTP	0.04 ± 0.001	20 ± 2	2 × 10−3	3 × 10−5