Fig. 4.

AM095 treatment decreases the number of dedifferentiating Schwann cells. Schwann cell dedifferentiation was investigated via immunohistochemistry by counting the number Sox2-positive cells in randomly selected fields on sciatic nerve sections. a + b A significant reduction of Sox2-positive cells with AM095 was observed at 21 dpi (N = 5/5). c + d This effect was even more pronounced at 28 dpi (N = 8/8). e + f In accordance with this finding, the number of differentiated Sox10-positive cells was significantly increased under treatment with AM095 at 28 dpi (N = 8/8). The number of Sox2/10-positive cells was related to the total number of cells stained with DAPI and normalized to vehicle-treated controls. Data represent normalized mean ± s.e.m. P ≤ 0.05*, P ≤ 0.01**. Scale bars indicate 50 µm