TABLE 2.

Gene conversion, BIR, and chromosome loss following an HO-induced DSB^a

Strain	Temp tested (°C)	No. tested	% Event for phenotype of colonies
			Ade+ Thr+ (GC)	Ade+ Thr+ Ade+ Thr− (GC + CO, GC + BIR)	Ade+ Thr− (BIR)	Ade+ Thr− Ade− Thr− (BIR + chrom. loss)	Ade− Thr− (chrom. loss)	Ade+ Thr+ Ade− Thr− (GC + chrom. loss)
Group A
Wild-type YLS100	30	638	70.6	28.0	1.0	0.3	0	0.1
	18	314	87.0	12.0	1.0	0	0	0
rad51Δ YLS101	30	706	2.0	0	13.0	70.0	15.0	0
	18	205	3.0	0	20.0	42.0	35.0	0
rad54Δ YLS102	30	383	1.0	0	14.0	30.0	55.0	0
	18	97	2.0	0	3.0	66.0	29.0	0
rad55Δ YLS103	30	78	55.0	10.0	3.0	10.0	5.0	17.0
	18	119	3.0	0	15.0	55.0	27.0	0
rad57Δ YLS104	30	764	52.0	13.0	4.0	8.0	8.0	15.0
	18	84	8.0	0	13.0	51.0	27.0	0
rad52Δ EI524b	30				3.0		97.0
Group B
sgs1Δ JZ604	30	155	90.0	3.9	4.1		2.0
sgs1Δ rad51Δ JZ605	30	187	5.9		34.2	26.7	33.2
Group C
rad1Δ YLS105	30	977	94.7	4.5	0.8
rad1Δ rad51Δ YLS106	30	641	2.0		27.9	21.9	45.2

^aDiploid cells illustrated in Fig. 1 were plated on YEP-Gal at the temperature indicated to induce expression of HO endonuclease, and colonies were then analyzed by replica plating to assess the retention of the ADE1 and THR4 alleles marking the left and right arms of chromosome III, respectively, as shown in Fig. 1. The types of events associated with each phenotypic class are also shown. GC, gene conversion. GC + CO, a sectored colony arising by reciprocal crossing over in G₂ cells. GC + BIR, a sectored colony showing both gene conversion and BIR. This category cannot be distinguished from GC + CO without further analysis. chrom. loss, chromosome loss. Sectored colonies with both gene conversion and loss (GC + chrom. loss) or BIR + chromosome loss (BIR + chrom. loss) are also indicated. 

^bData for rad52Δ diploid EI524 are taken from Malkova et al. (38).