Fig. 7.

CD147-CART cells show potent anti-tumor activity against NSCLC in PDX models. A Schematic representation of PDX model treatment. B, C The dynamic curves of tumor volume and mouse weight were measured. Data are presented as mean ± SEM (*P < 0.05, n = 3). D, E The mice appearance and tumor morphology were shown. F, G The tumor weight and tumor volume were quantified in PBMC group and CD147-CART group. Data are presented as mean ± SEM (*P < 0.05, n = 3). H The cell apoptosis in tumor tissues was evaluated using TUNEL Assay Kit; Scale bar = 200 µm. I Ki67-positive tumor cells in tumor tissues were evaluated by IHC staining; Scale bar = 100 µm. J Ki67-positive tumor cell numbers in 12 random fields (12 dots from 3 mice, 4 dots/ mouse) in each group were counted and analyzed. Data are presented as mean ± SEM (***p < 0.001, n = 12 independent experiments). K, L CD3+ T cells (k) and CD8+ T cells (l) in tumor tissues were evaluated by IHC staining; Scale bar = 100 µm.