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Two-hybrid analysis of Ume6 interactions, using an rpd3Δ derivative of PJ69-4A containing GBD-Ume6 baits and either GAD-Sin3 or GAD-Tea1 library clones. (A) Interaction between Ume6 and library clones is shown by growth on Ade− medium resulting from expression of the GAL2-ADE2 reporter. (B) Interaction between GBD-Ume6 with deletion derivatives of GAD-Sin3, shown by growth on His− medium resulting from expression of the GAL1-HIS3 reporter.
