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Sporulation of strains containing fusions of the Gal4 activation domain to UME6 or ume6-6. A wild-type diploid (W303a/α; lanes 1 to 3) or ime1Δ diploid (REE2276/REE3086; lanes 4 to 6) was transformed with GAD, GAD-UME6, or GAD–ume6-6 plasmids. Transformants were replica plated to sporulation medium, and asci were counted after 5 days at 37°C.
