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Complementation of a ume6Δ diploid by UME6 plasmids. Vegetative derepression and sporulation in a ume6Δ diploid (YC105/YC121) containing pBK8 and either vector (pRS315; lane 1), wild-type (WT) UME6 plasmid (pPL5905; lane 2), or mutant derivatives of pPL5905 containing the indicated mutations in the α helix (lanes 3 to 6). Lanes 4 and 5 represent two independent isolates of the L527P mutation.
