FIG. 5.

The EGF-R2A mRNA sequence acts as a cis destabilizing and EGF-regulated element. MDA-MB-468 cells were transfected with pRSV-Luc or pRSV-Luc/EGF-R2A in triplicate. After treatment with EGF (25 ng/ml) for 6 h, the cells were treated with Act D (7.5 μg/ml), and RNA was extracted at 0, 2, 4, and 8 h followed by RT-PCR for Luc mRNA using a LightCycler (see Materials and Methods). Samples were normalized using a cotransfected control of β-globin. Half-lives were determined by linear regression analysis. The graph is representative of data from four independent experiments in duplicate. Rel., relative.