FIG. 6.

Isw2 and Itc1 function in the same pathways. (A) Deletion of ISW2 or ITC1 results in derepression of meiotic genes. Northern blot analysis was performed to compare expression levels of meiotic genes in vegetatively growing cells. The genotype of cells used is listed above each lane; the gene probed is indicated at the right. Numbers below the lanes represent levels of transcription in mutants relative to wild-type cells. ACT1 was used as a loading control. WT, wild type. (B) isw2 and itc1 mutations confer temperature-sensitive growth defects in combination with isw1 and chd1 mutations. Tenfold serial dilutions of saturated wild-type and mutant liquid cultures were spotted onto rich medium (YEPD) and incubated at 30, 37, or 38.5°C.