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The steady-state kinetics of CdgSH and its variants CdgSHGGAAF (abolishing the synthesis activity) and CdgSHAAA (abolishing the hydrolysis activity).
| Enzyme | DGC (GTP→c-di-GMP) | PDE (c-di-GMP→pGpG) | Km (μM) | Kcat (s−1) | Kcat/Km (μMs−1) | Km (μM) | Kcat (s−1) | Kcat/Km (μMs−1) | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CdgSH | 37.2 | 0.39 | 1.05×10−2 | 23.5 | 1.26 | 5.4×10−2 | ||||||
| CdgSHGGAAF | — | — | — | 31.0 | 1.78 | 5.7×10−2 | ||||||
| CdgSHAAA | 34.8 | 0.12 | 3.45×10−3 | — | — | — | ||||||
