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. 2021 Dec 6;49(22):13179–13193. doi: 10.1093/nar/gkab1170

Figure 4.

Figure 4.

GCPANFab triplex X-ray crystal structure. (A) Secondary structure of GCPAN triplex, whose apical GAAA tetra-loop was replaced by the Fab-BL3-6 binding motif (5′-GAAACAC-3′, depicted in outlined font). (B) Crystal structure of GCPAN triplex shown in stick representation and the 3.3 Å anneal-omit 2|Fo| – 2|Fc| electron density map (blue mesh) of the RNA molecule contoured at 1.5 σ level (PDB ID: 6X5N). (C) Top view of GCPAN triplex crystal structure model, depicting the P2-triplex-P1 co-axial stack. (D) Model of the five U•A–U base triples in the triplex region colored in blue (U), orange (A) and green (U). (E) A representative U•A–U base triple. (F) A representative A-minor interaction between gcc(A)9 and the P1 duplex.