Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 1;49(22):12929–12942. doi: 10.1093/nar/gkab1152

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — PRC5 is part of a complex with PRP19-related proteins and the trypanosomatid-specific protein PRC3. (A) The final eluate of PRC5-PTP tandem affinity purification was sedimented through a 10–40% linear sucrose gradient by ultracentrifugation and fractionated from top to bottom into 20 aliquots. Proteins from each fraction were separated by SDS-PAGE and stained with SYPRO Ruby. Protein bands were excised and identified by LC/MS/MS. For comparison, sedimentations of TEV protease (29 kDa), Taq DNA polymerase (95 kDa), IgG (150 kDa, 6.6S) and apoferritin (444 kDa, 17S), were analyzed in parallel gradients (arrowheads). (B) Schematic representation to scale of the five PRC subunits. For comparison, the human SYF1, SYF3 and ISY1 homologs are depicted, too. Pink, green and gray boxes indicate TPR domains, ISY1 domains and highly conserved regions among trypanosomatid homologs, respectively.