Figure 5.

The predominant Mre11-dependent incision shows sequence dependence more than a strict positional dependence. (A) Diagram of a 3 bp deletion in ILV1 located 31–33 bp from the HOcs. (B) Fractional resection signal from positions −20 to −100 in WT and exo1Δ yeast 90 min after DSB induction with and without the 3 bp deletion at positions −31 to −33. Vertical dashed lines in the top plot mark the location of the positions that were deleted in the bottom plot. A vertical blue line indicates the position expected for the peak in the bottom plot if the distance from the DSB end had been maintained after the 3 bp deletion. (C) Diagram of a 3 bp deletion in ILV1 located 71–73 bp from the HOcs. (D) Similar to (B) for the 3 bp deletion located at positions −71 to −73.