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. 2021 Dec 6;49(22):13122–13134. doi: 10.1093/nar/gkab1190

Table 1.

DNA and RNA sequences used in this study

Sequence name	Sequence (5′ → 3′)	Notes
VmeRepeat	GTTCACTGCCGCACAGGCAG/CTTAGAAA	RNA cleavage site indicated by/
pUC spacer	gaattcgagctcggtacccggggatcctctag	pUC targeting spacer
pUC target	GACUcuagaggauccccggguaccgagcucgaauucAAAGAUUC	synthetic RNA target
N gene N57 spacer^a	cattctggttactgccagttgaatctgagggt	SARS-CoV-2 N gene targeting spacer
N gene N209 spacer^a	tggactgctattggtgttaattggaacgcctt	SARS-CoV-2 N gene targeting spacer
N gene N320 spacer^a	tggcccagttcctaggtagtagaaataccatc	SARS-CoV-2 N gene targeting spacer
N gene N719 spacer^a	agatttcttagtgacagtttggccttgttgtt	SARS-CoV-2 N gene targeting spacer
N gene N782 spacer^a	tgttacattgtatgctttagtggcagtacgtt	SARS-CoV-2 N gene targeting spacer
N gene N1169 spacer^a	caaatctgcagcaggaagaagagtcacagttt	SARS-CoV-2 N gene targeting spacer
N57 target	GUGGacccucagauucaacuggcaguaaccagaaugGAGAACGC	Synthetic RNA
N209 target	GGACaaggcguuccaauuaacaccaauagcaguccaGAUGACCA	Synthetic RNA
N209(+3) target	GGACaaggcguuccaauuaacaccaauagcaguGcaGAUGACCA	Synthetic RNA
N719 target	CAACaacaacaaggccaaacugucacuaagaaauctGCUGCUGA	Synthetic RNA
N782 target	CAAAaacguacugccacuaaagcauacaauguaacaCAAGCUUU	Synthetic RNA
Non-target RNA (NTR)	aggguauuauuuguuuguuucuucuaaacuauaagcuaguucuggaga	Synthetic RNA
NucC substrate	agtgttacattatccaccatggcgagcttt	Synthetic dsDNA^b

^aThe number indicates the position of the target sequence in the SARS-CoV-2 N gene.

^b5′-FAM on top strand, 3′-Iowa Black® on bottom strand.