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. 2021 Dec 9;49(22):12986–12999. doi: 10.1093/nar/gkab1204

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Q levels in tRNA decrease in response to high tyrosine concentration in the growth media. The figure shows the results of growing cells in media containing ‘high tyrosine’, where total RNA was isolated at various time points in the growth curve as indicated. (A) shows a representative APB-gel northern blot using radioactive oligonucleotide probes specific for the four Q-containing tRNAs. ‘Q34’, ‘G34’ and ‘ox’ refer to Q-modified, unmodified and the oxidized control respectively as in previous figures. (B) quantification of the results from (A), where percent Q modified tRNA was calculated as described before. The graph is the result of three independent experiments.