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. 2021 Dec 9;49(22):12986–12999. doi: 10.1093/nar/gkab1204

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 8. — Intracellular concentrations of free q and Q are not altered by tyrosine concentration. The figure shows accurately quantified levels of free q and Q as determined by a specific LC–MS/MS method employing a 7-point calibration curve. LTC, HTC and CC refer to cells grown in low tyrosine, high tyrosine or complete media as indicated. (A) shows the levels of free q intracellularly and in media along with the cellular q expressed as a % of total q, (B) shows corresponding data for free Q. Data were compared by One-way analysis of variance (ANOVA) with significant differences indicated (*P< 0.05, **P< 0.01, ***P< 0.001 and ^****P< 0.0001).