Figure 9.

Q changes in tRNA driven by fluctuations in tyrosine concentrations affect decoding of the U-ending codons for tyrosine. A dual-luciferase reporter assay where a translational frameshifting window is used to assess the efficiency of decoding the U-ending or C-ending codons for tyrosine in response to changes in media tyrosine concentration. (A) a schematic of the assay where the test codon was either UAA or UAU for tyrosine. Non-Q requiring cysteine codons were used as a control to highlight the Q dependence. The ‘in-frame’ constructs, which lack a frameshifting window, were used for normalization. (B) the results of 3 independent experiments. L, C and H refer to cells expressing the dual-luciferase reports and grown in either ‘low’, ‘complete’ or ‘high’ tyrosine media.