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. 2021 Dec 8;49(22):12769–12784. doi: 10.1093/nar/gkab1146

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Head-on and co-directional replication-transcription conflicts in WT and sen1 strains. (A) 2D gel analysis of replication intermediates at the PDC1 gene undergoing head-on (HO) (Left Panel) or co-directional (CD) (Right Panel) collisions with ARS607-dependent replication upon digestions with PstI (P)/BamHI (B) or NdeI (N), respectively. (B) RNA:DNA hybrid levels at PDC1 gene analyzed by DRIP-qPCR. Data represent mean ± SEM from three independent experiments. ns, not significant; **P < 0.01 (two-way ANOVA). (C) ChIP-qPCR analysis at PDC1 locus of H2A-S139 phosphorylated (γ-H2AX). Data represent mean ± SEM from three independent experiments. ****P < 0.0001 (two-way ANOVA). (D) Serial dilutions of the indicated yeast strains spotted on YPD plates with or without NaN3 grown at the semi-permissive temperature of 30°C for sen1-1.