Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 6;49(22):13045–13061. doi: 10.1093/nar/gkab1148

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 1. — HDNMT2 catalyzes m⁵C38 modification on tRNA^Gly(GCC) in vitro. (A) SDS-PAGE analysis of the purified recombinant hDNMT2. Standard molecular weights are shown on the left. (B) The purified hDNMT2 was analyzed by gel filtration chromatography on a Superdex™ 200 column. HDNMT2 was eluted at 15.5 ml. The evolution volume of the standard proteins was marked above the graph. (C) The secondary structures of tRNA^Gly(GCC), tRNA^Asp(GUC), and tRNA^Val(.AC). (D) The capacity of tRNA^Gly(GCC) and the three mutants which were generated by in vitro transcription: -C38A, -C38G, -C38U to be methylated by hDNMT2. (E) tRNA^Gly(GCC) and the -C38G, and -C38A mutants incubated with hDNMT2 analyzed by UPLC-MS/MS analysis after digestion. Chromatogram of m⁵C (Q1/Q3 = 258.1/126.1) and A (Q1/Q3 = 268.1/136.2) are described, respectively. Error bars represent the standard errors of three independent experiments in Figures 1–8.