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The D-stem and variable loop are recognized by hDNMT2. (A, B) Mutants of the D-stem region. The capacity of tRNAGly(GCC)-U11C:A24G (A) and -U12G:A23C (B) to be methylated by hDNMT2. (C) Mutants of the variable loop. The capacity of tRNAGly(GCC) with several mutations in variable loop to be methylated by hDNMT2.
