Table 3.

Single-turnover experiments with hNTHL1Δ63, hNTHL1Δ63 chimera, and full-length hNTHL1. The rate constants are reported in this table. The rate constant k₂ is the observed rate of glycosylase only activity, quenched with NaOH. The rate constant k₃ is the observed rate of β-elimination activity, quenched with formamide stopping dye. Experiments were repeated three times. The rate constants were calculated by fitting the data to a one-phase association exponential, Y = Y0 + (plateau-Y0) * (1 − exp(−K*x)).

		Tg:A
	k 2 (min–1)	99% CI	k 3 (min–1)
hNTHL1Δ63	2.95	1.53–5.65	0.45
hNTHL1Δ63 Chimera	0.070	0.021–0.15	n/a
hNTHL1 FL	4.53	3.40–6.35	–