. 2021 Dec 6;49(22):12785–12804. doi: 10.1093/nar/gkab1156

Table 1.

DDC2 synthetic dosage lethality genes

Genes with epsilon < –0.08 in at least 2 replicates^a	Systematic name	Replicate 1 epsilon^b	Replicate 2 epsilon	Replicate 3 epsilon	Mean epsilon	Std Dev	Brief description^c
YCR102C	YCR102C	−0.34593	−0.26015	−0.22037	−0.2755	0.0524	Putative quinone oxidoreductase
GAL80	YML051W	−0.30714	−0.16683	−0.25393	−0.2426	0.0578	Transcriptional regulator involved in the repression of GAL genes
PYC2	YBR218C	0	−0.38475	−0.23274	−0.2058	0.1582	Pyruvate carboxylase isoform
PMA2	YPL036W	−0.25367	−0.11122	−0.1438	−0.1696	0.0609	Plasma membrane H+-ATPase
RAD27	YKL113C	−0.16946	−0.08576	−0.16575	−0.1403	0.0386	5′ to 3′ exonuclease, 5′ flap endonuclease
VID22	YLR373C	−0.07129	−0.10449	−0.21956	−0.1318	0.0635	Glycosylated integral membrane protein localized to plasma membrane
MMS22	YLR320W	−0.13772	−0.00623	−0.23168	−0.1252	0.0925	Subunit of E3 ubiquitin ligase complex involved in replication repair
VPS68	YOL129W	−0.02521	−0.23802	−0.10864	−0.1240	0.0876	Vacuolar membrane protein of unknown function
THR4	YCR053W	−0.12709	−0.11031	−0.12391	−0.1204	0.0073	Threonine synthase
DBP7	YKR024C	−0.08875	−0.16635	−0.10059	−0.1186	0.0341	Putative ATP-dependent RNA helicase of the DEAD-box family
SLX5	YDL013W	−0.112	−0.1192	−0.12084	−0.1173	0.0038	Subunit of the Slx5-Slx8 SUMO-targeted Ub ligase (STUbL) complex
LRO1	YNR008W	0.00822	−0.15022	−0.19615	−0.1127	0.0875	Acyltransferase that converts diacylglycerol to triacylglycerol (TGA)
NKP2	YLR315W	−0.21546	−0.02581	−0.09278	−0.1114	0.0785	Central kinetochore protein and subunit of the Ctf19 complex
NPL4	YBR170C	−0.10522	−0.12311	−0.10379	−0.1107	0.0088	Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase
SST2	YLR452C	−0.15523	−0.1565	−0.01984	−0.1105	0.0641	GTPase-activating protein for Gpa1p
IRC4	YDR540C	−0.1842	−0.02255	−0.106	−0.1043	0.0660	Protein of unknown function
RPL22A	YLR061W	−0.12007	0.00518	−0.19649	−0.1038	0.0831	Ribosomal 60S subunit protein L22A
SCS22	YBL091C-A	−0.09108	−0.07698	−0.13178	−0.0999	0.0232	Protein involved in regulation of phospholipid metabolism
EKI1	YDR147W	−0.12455	−0.07955	−0.09191	−0.0987	0.0190	Ethanolamine kinase
SLX8	YER116C	−0.15284	0.01681	−0.15558	−0.0972	0.0806	Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex
MNN10	YDR245W	−0.09849	−0.11437	−0.07617	−0.0963	0.0157	Subunit of a Golgi mannosyltransferase complex
PTC4	YBR125C	−0.23321	−0.11234	0.05822	−0.0958	0.1196	Cytoplasmic type 2C protein phosphatase (PP2C)
RTG3	YBL103C	−0.09982	−0.08377	−0.09895	−0.0942	0.0074	bHLH/Zip transcription factor for retrograde (RTG) and TOR pathways
PAC10	YGR078C	0.0254	−0.13636	−0.16526	−0.0921	0.0839	Part of the heteromeric co-chaperone GimC/prefoldin complex
SLX9	YGR081C	−0.0811	−0.08407	−0.10933	−0.0915	0.0127	Protein required for pre-rRNA processing
GUP1	YGL084C	−0.02287	−0.15367	−0.09719	−0.0912	0.0536	Plasma membrane protein involved in remodeling GPI anchors
KCC4	YCL024W	−0.13964	−0.08448	−0.04869	−0.0909	0.0374	Protein kinase of the bud neck involved in the septin checkpoint
MRS2	YOR334W	−0.1369	−0.15801	0.02559	−0.0898	0.0820	Mitochondrial inner membrane Mg(2+) channel
RTS1	YOR014W	−0.06842	−0.09715	−0.09979	−0.0885	0.0142	B-type regulatory subunit of protein phosphatase 2A (PP2A)
TIM18	YOR297C	0.09083	−0.14955	−0.20461	−0.0878	0.1283	Component of the mitochondrial TIM22 complex
SAC7	YDR389W	−0.09922	−0.07025	−0.08967	−0.0864	0.0121	GTPase activating protein (GAP) for Rho1p
HTZ1	YOL012C	−0.12843	−0.09077	−0.03809	−0.0858	0.0371	Histone variant H2AZ
CTF4	YPR135W	−0.00526	−0.10066	−0.14951	−0.0851	0.0599	Chromatin-associated protein
LYS2	YBR115C	0	−0.13134	−0.1208	−0.0840	0.0596	Alpha aminoadipate reductase
YDL162C	YDL162C	−0.01668	−0.08016	−0.1529	−0.0832	0.0557	Overlaps with CDC9 promoter
MRC1	YCL060C	−0.10752	−0.05301	−0.08651	−0.0823	0.0224	S-phase checkpoint protein required for DNA replication
PHO5	YBR093C	−0.14925	−0.12743	0.03574	−0.0803	0.0825	Repressible acid phosphatase
MRX10	YDR282C	−0.09352	−0.0926	−0.04064	−0.0756	0.0247	Mitochondrial inner membrane protein of unknown function
RPL40A	YIL148W	0.10568	−0.16715	−0.16321	−0.0749	0.1277	Ubiquitin-ribosomal 60S subunit protein L40A fusion protein
XDJ1	YLR090W	0.0167	−0.08185	−0.15132	−0.0722	0.0689	Chaperone with a role in facilitating mitochondrial protein import
YNL140C	YNL140C	−0.02494	−0.08911	−0.10192	−0.0720	0.0337	Protein of unknown function
PEX9	YMR018W	−0.09397	−0.09198	−0.02484	−0.0703	0.0321	Peroxisomal membrane signal receptor for peroxisomal matrix proteins
UBP6	YFR010W	−0.09206	−0.00378	−0.11453	−0.0701	0.0478	Ubiquitin-specific protease
DST1	YGL043W	−0.09769	0.00767	−0.11659	−0.0689	0.0547	General transcription elongation factor TFIIS
SPO73	YER046W	−0.12322	−0.09846	0.02037	−0.0671	0.0627	Meiosis-specific protein required for prospore membrane morphogenesis
BRE5	YNR051C	−0.13273	−0.1059	0.0386	−0.0667	0.0752	Ubiquitin protease cofactor
MCA1	YOR197W	−0.0821	−0.10252	−0.00329	−0.0626	0.0428	Ca²⁺-dependent cysteine protease
SIF2	YBR103W	0.01601	−0.09958	−0.09643	−0.0600	0.0538	WD40 repeat-containing subunit of Set3C histone deacetylase complex
TOS4	YLR183C	0.05837	−0.12147	−0.10298	−0.0554	0.0808	Putative transcription factor, contains Forkhead Associated domain
YML090W	YML090W	0.04237	−0.09205	−0.11481	−0.0548	0.0694	Dubious open reading frame
SCS7	YMR272C	0.20929	−0.15347	−0.0807	−0.0083	0.1567	Sphingolipid alpha-hydroxylase
ASK10	YGR097W	0.59527	−0.11235	−0.10438	0.1262	0.3317	Regulator of the Fps1p glycerol channel

^aGenes identified in all three replicates are indicated in bold.

^bEpsilon values with P > 0.05 are entered as ‘0’.

^cAs annotated in YeastMine (https://yeastmine.yeastgenome.org/).