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. 2021 Dec 6;49(22):13075–13091. doi: 10.1093/nar/gkab1161

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Inference of a sequence signature and folding potential around cleavage sites. (A) Analysis of minimal folding energy (ΔG) around detected cleavage sites, start and stop codons and annotated starts and ends of transcriptional units. Minimal folding energy was calculated at each nucleotide position using a sliding window of 25 nt and a 1 nt step size for a segment of 150 nt up- and downstream of the respective element. (B) Sequence logos of PSS accumulating in rne(WT) or rne(Ts) after heat treatment. Sequences were aligned according to the detected cleavage site. Error bars were calculated by the WebLogo tool and represent an approximate Bayesian 95% confidence interval. (C) Nucleotide composition around detected PSS.