Table 1. Binding isotherm and signaling properties.

Binding in intact cells was performed with PTH_1–34^TMR and the binding affinity constant K_i calculated after the Cheng and Prusoff correction. For R⁰ and R^G, the binding was performed in plasma membrane extracts from HEK293 cells lacking Gs by CRISPR-Cas9 deletion and transiently transfected with PTHR without (R⁰) or with (R^G) Gs-ND. Data are means ± SEM from N = 3 (binding), N = 4 (cAMP), and N = 5 (β-arr1 and β-arr2) independent experiments.

Ligands	Binding Intact cells		Binding R0		Binding RG
	Ki (nM)	P	IC50 (nM)	P	IC50 (nM)	P
PTHWT	4.97 ± 0.92	1.00	4.70 ± 1.33	1.00	0.10 ± 0.04	1.00
PTH7d	4.27 ± 0.80	1.00	17.14 ± 3.28	0.015	0.10 ± 0.04	1.00

Ligands	cAMP		βarr1		βarr2
	pEC50	Emax	pEC50	Emax	pEC50	Emax
PTHWT	9.36 ± 0.04	100	8.32 ± 0.04	100	8.37 ± 0.05	100
PTH7d	9.45 ± 0.08	99 ± 5	7.76 ± 0.08	40 ± 2	7.71 ± 0.11	37 ± 3